Figure 2
From: Structural analysis of polarizing indels: an emerging consensus on the root of the tree of life

HisA does not exclude the root from the Eobacteria. A MUSCLE based alignment of all the HisA sequences in Eobacteria. Represenatives from Actinobacteria (Streptomyces coelicolor A3) and other Gram-negatives (Synechocystis sp. PCC 6803) are included to show the indel. All the Eobacterial sequences share the relative deletion with the Firmicutes (Bacillus clausii).