Figure 4

Foxp3 expression in different subsets of splenocytes. Splenocytes were stained with antibodies against CD4 (PE-Cy7), CD8 (FITC), CD44 (PE), CD25 (APC-Alexa Fluor755), Foxp3 (PE-Cy5) and analyzed by flow cytometry. (A) Foxp3 expression (%) in CD4⁺CD25⁺ cells that was either positive or negative for CD44. (B) Foxp3 expression (%) in CD4⁺CD25⁺ cells that expressed high, medium, or low levels of CD44. Shown are representative results with mean ± S.D. from three independent experiments (** p < 0.01, *** p < 0.001).