Fig. 4

NISCH inhibits the proliferation and migration of GC cells. A QPCR was performed to detect the overexpression efficiency of pcDNA3.1-NISCH in AGS and NUGC4 cells. B–E CCK-8, EdU, wound healing and transwell assays were used to evaluate the proliferation and migration of AGS and NUGC4 cells after the overexpression of NISCH. The statistical analysis for A, C, D, E was student’s t-test, and for B was two-way ANOVA. β-actin was used as the internal reference for gene expression analysis. **P < 0.01