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Fig. 2 | Biology Direct

Fig. 2

From: Upregulation of long intergenic non-coding RNA LINC00326 inhibits non-small cell lung carcinoma progression by blocking Wnt/β-catenin pathway through modulating the miR-657/dickkopf WNT signaling pathway inhibitor 2 axis

Fig. 2

LINC00326 silencing promotes proliferation and reduces apoptosis in NSCLC cells. a Relative expression of LINC00326 in NSCLC and normal BEAS-2B cells, measured by RT-qPCR and normalized to controls (equal to 1). b Subcellular localization of LINC00326 in A549 and HCC827 cells measured by the isolation of cytoplasmic and nuclear RNA followed by RT-qPCR analysis. c Transfection efficiencies of sh-LINC00326 and OE-LINC00326 in A549 and HCC827 cells, measured by RT-qPCR. d–f Proliferation of transfected A549 and HCC827 cells, measured by CCK-8 and Edu assays. g–h Apoptosis of transfected A549 and HCC827 cells, measured by flow cytometry. Data are presented as mean ± standard deviation. i Wnt1, Wnt3a, and β- catenin protein levels in transfected A549 and HCC827 cells, shown by Western blotting. The experiments were repeated three times. * P < 0.05; ** P < 0.01; *** P < 0.001

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