Fig. 6

After the overexpression of MYH9, the effect of knocking down TM4SF1 on the cancer stemness of HCC cells could be recovered. A Results of the WB analysis showed that after the overexpression of MYH9, the decrease in the molecules related to the NOTCH pathway and cancer stemness caused by knocking down TM4SF1 could be restored in HCC cells. B Results of the experiment for the detection of side-population cells showed that after the overexpression of Myh9, the decrease in the proportion of stem cells caused by TM4SF1 knockout could be restored. C Experiment for the tumor sphere formation showed that after the overexpression of Myh9, the reduction in the number and size of tumor spheres caused by the silencing of TM4SF1 could be restored. Scale bars, 50 μm. D Comparison of the number of tumor spheres and the proportion of tumor spheres with different diameters