Skip to main content
Fig. 2 | Biology Direct

Fig. 2

From: Deep learning-assisted high-content screening identifies isoliquiritigenin as an inhibitor of DNA double-strand breaks for preventing doxorubicin-induced cardiotoxicity

Fig. 2

Construction of a high-content imaging method for doxorubicin-induced DNA damage by labeling with fluorescent protein. A Map of the lentiviral vector for a stable expression of EGFP-53BP1 (1220 ~ 1711 aa). B Representative two-color fluorescent images of EGFP-53BP1-H9c2. C Distribution of 53BP1 after the treatment of EGFP-53BP1-H9c2 with 0.5 μM DOX for different time durations. D The experimental images in (C) were analyzed by the traditional method. Data are plotted as mean ± SD, n = 3, *P < 0.05, **P < 0.01 when compared with the control group. E Effect of dexrazoxane (DXZ) or resveratrol (RES) on the distribution of 53BP1 in EGFP-53BP1-H9c2 treated with 0.5 μM DOX for 1 h

Back to article page

Biology Direct

ISSN: 1745-6150

Contact us