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Fig. 2 | Biology Direct

Fig. 2

From: Depletion of LONP2 unmasks differential requirements for peroxisomal function between cell types and in cholesterol metabolism

Fig. 2

Cell-specific ISR activation upon silencing of LONP2. RNA sequencing (n = 3 independent experiments per condition), LONP2 silencing for 7 days. a Principal component (PC) score plot. b Volcano plots showing all identified transcripts. c Transcripts encoding integrated stress response (ISR) factors. d Immunoblotting for ISR-related proteins, vinculin was used as a loading control. e Translation assay: cells were incubated with puromycin for 10 min., and incorporation of puromycin into newly synthesized proteins was revealed with anti-puromycin antibodies. GAPDH was used as a loading control. f Transcripts encoding ribosomal biogenesis-related rRNA, snoRNA and mRNA genes. g Immunoblot of ribosomal biogenesis regulator RRS1 in NT- and LONP2-silenced COS-7 cells and U2OS cells

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Biology Direct

ISSN: 1745-6150

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