Fig. 3

LONP2 silencing leads to impaired retinoic acid signaling. a Gene ontology of RNA sequencing data (dataset is described in Fig. 2). Numbers on Venn diagram indicate the number of genes in each category. b Immunoblotting of retinoic acid regulating proteins, vinculin used as a loading control. c COS-7 or U2OS cells were incubated in the presence or absence of 5 µM retinoic acid for 24 h and mRNA transcript levels for LONP2 (left two panels) and TGM2 (right two panels) quantified with qRT-PCR. n = 3 independent experiments, means are depicted in different colours. The mean values were used to calculate the average (horizontal bar), s.d. (error bars) and P-values (using a two-tailed unpaired Student’s t-test)