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Fig. 3 | Biology Direct

Fig. 3

From: Overexpression of ZNF488 supports pancreatic cancer cell proliferation and tumorigenesis through inhibition of ferroptosis via regulating SCD1-mediated unsaturated fatty acid metabolism

Fig. 3

ZNF488 regulates apoptosis and ferroptosis in pancreatic cancer cells. A, B siCtrl, siZNF488#1 and siZNF488#2 MIAPaCa-2 and PANC-1 cells were subjected to PI and Annexin V staining which was analyzed on flow cytometry. C Caspase 3/caspase 7 activity was measured. D, E siCtrl, siZNF488#1 and siZNF488#2 MIAPaCa-2 and PANC-1 cells were treated with DMSO, erastin (5 µM), combining with or without ferrostatin-1 (2 µM) or Z-VAD-FMK (8 µg/ml). Cell viability was analyzed by trypan blue. F-H Ctrl and ZNF488 overexpressing MIAPaCa-2 and PANC-1 cells were treated with DMSO or erastin (5 µM), combining with or without ferrostatin-1 (2 µM). Then the cells were subjected to PI staining and trypan blue staining. *p < 0.05. **p < 0.01

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