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Fig. 2 | Biology Direct

Fig. 2

From: Effect of glycosylation on the affinity of the MTB protein Ag85B for specific antibodies: towards the design of a dual-acting vaccine against tuberculosis

Fig. 2

(a) Chemical structure of the different glycans activated with IME linker. (b) Chemical glycosylation of proteins. Reactions were performed in 100 mM sodium tetraborate pH 9.5 at 25 °C for 24 h; glycosidic reagent/protein molar ratio: 250:1 for Ara3Man-rAg85B-K30R/K282R, and 200:1 for the others; protein concentration: 2 mg/mL. In rAg85B and rAg85-K30R/K282R glycosylation, 1mM benzamide hydrochloride was added in the reaction mixture to avoid potential proteolysis [33]. Protein graphical representation depicts Ag85B protein and was taken from the Protein data Bank (PDB DOI: https://doiorg.publicaciones.saludcastillayleon.es/10.2210/pdb1f0p/pdb)

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