Fig. 6

USP40 promotes HCC progression by regulating Claudin1. A Knockdown of USP40 decreased Claudin1 protein levels, and this effect was reversed by Claudin1 overexpression. Claudin1 or vector plasmids were transfected into MHCC97H cells with stable USP40 knockdown, and expression of USP40 and Claudin1 was detected by western blotting. The proliferative and migratory capacity of MHCC97H cells was detected by CCK-8 (B), colony formation (C), wound healing (D) and transwell (E), respectively. F SiClaudin1 or siNC were transfected into MHCC97H cells that USP40 stably overexpressed. USP40 and Claudin1 expression was detected by western blotting. The proliferative and migratory ability of MHCC97H cells was also tested by CCK-8 (G), colony formation (H), wound healing (I) and transwell (J), respectively. Data were displayed with typical pictures of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001