Fig. 1

Dox downregulates NSUN2 levels in liver cells. a The morphology of LO2 cells after 24 h of Dox treatment (n = 3, scale bar = 75 µm). b The effect of Dox treatment on LO2 cell vitality (n = 3). c Western detection of the effect of Dox treatment on NSUN2 expression in LO2 cells (n = 3). d Western detection of the effect of Dox treatment on the expression of NSUN2 in primary liver cells (n = 3). e Representative images of liver tissue HE staining in the mice (1, 4, and 6 day) under a microscope (n = 3, scale bar = 20 µm). f Serological detection of ALT and AST (n = 5). g Immunohistochemical detection of liver NSUN2 expression (n = 3, scale bar = 20 µm). h Western detection of NSUN2 expression in liver tissue (n = 3). The data is represented by the average ± SD of three trials, and compared with the control group, *P < 0.05, **P < 0.01, ***P < 0.001