Fig. 2

NSUN2 knockout promotes Dox-induced LO2 cell damage. a Western blot detection of the expression of NSUN2 in LO2 cells after Dox-induced injury (n = 3). b Flow cytometry detection of the apoptosis level of LO2 cells following Dox-induced injury (n = 3). c HE detection of pathological changes in 3 days after Dox-induced liver injury (n = 3, scale bar = 20 µm). d Serological detection of ALT and AST in 3 days after Dox-induced liver injury (n = 6). e Immunohistochemical detection of NSUN2 expression in the liver of AAV9-shNSUN2 liver injury mice (n = 3, scale bar = 20 µm). f Western blot detection of NSUN2 expression in liver tissues of each group in 3 days after Dox-induced liver injury (n = 3). g TUNNEL detection of liver apoptosis level in shNSUN2 liver injury mice (n = 3, scale bar = 20 µm). The data is represented by the average ± SD of three trials, and compared with the control group, *P < 0.05, **P < 0.01, ***P < 0.001