Fig. 3

Overexpression of NSUN2 alleviates Dox-induced liver damage. a Western detection of NSUN2 expression in LO2 and liver primary cells after Dox treatment (n = 3). b Flow cytometry detection of the level of apoptosis in LO2 cells overexpressing NSUN2 following Dox-induced injury (n = 3). c HE detection of pathological changes in Dox-induced liver injury in AAV9-NSUN2 mice (n = 3, scale bar = 20 µm). d Serological detection of ALT and AST in 3 days after Dox treatmen (n = 3). e Immunohistochemical detection of NSUN2 expression in the liver of AAV9 AdNSUN2 liver injury mice 3 days after Dox treatment (n = 3, scale bar = 20 µm). f TUNEL detection of apoptosis cells in AAV9-AdNSUN2 liver injury mice in 3 days after Dox treatment (n = 3, scale bar = 20 µm). The data is represented by the average ± SD of three trials, and compared with the control group, *P < 0.05, **P < 0.01, ***P < 0.001