Fig. 4

NSUN2 downregulates Nrf2 and promotes Dox-induced oxidative stress response in liver cells. a Immunohistochemical staining detected Nrf2 expression in the liver of mice treated with saline or Dox (n = 3, scale bar = 20 µm). b Western detection of Nrf2, NQO1, and HO-1 expression in LO2 cells treated with different concentrations of Dox (n = 3). c Western detection of Nrf2, NQO1, and HO-1 expression in liver primary cells after Dox treatment (n = 3). d Western detection of Nrf2, NQO1, and HO-1 expression in liver tissues after 1, 4 and 6 days of Dox treatment (n = 3). e Western detection of Nrf2, NQO1, and HO-1 expression in WT or NSUN2-Cas9 LO2 cells treated with 2 µM of Dox. f Western detection of Nrf2, NQO1, and HO-1 expression in control or shNSUN2 mice liver tissues 3 days after 20 mg/kg Dox treatment (n = 3). g Flow cytometry detection of NSUN2 knockdown on ROS levels in LO2 cells. The data is represented by the average ± SD of three trials, and compared to the control group, *P < 0.05, **P < 0.01, ***P < 0.001. Compared to vector Dox group, ^#P < 0.05, ^##P < 0.01