Fig. 5
Effect of NSUN2 on Nrf2 mRNA. a RT-PCR detection the half-life of Nrf2 mRNA in LO2 cells of each group after actinomycin treatment (n = 3). b RIP detection the combination and recognition ability of NSUN2 and Nrf2 mRNA (n = 3). c Western blot analysis of the effect of NSUN2 wild-type or mutant plasmids on Nrf2 protein level (n = 3). d RNA fluorescence probe detection of the effect of NSUN2 overexpression on Nrf2 mRNA (n = 3, scale bar = 10 µm). e Firefly luciferase detection of the binding region between NSUN2 and Nrf2 (n = 3). The data is represented by the average ± SD of three trials, and compared with the control group, *P < 0.05, **P < 0.01, ***P < 0.001