Fig. 6

ALYREF recognizes and binds Nrf2 mRNA. a RIP detection of the combination and recognition ability of ALYREF and Nrf2 mRNA (n = 3). b Western detection of NSUN2, Nrf2, and ALYREF protein expression in each group (n = 3). c Western blot detection of the expression of NSUN2, Nrf2, and ALYREF proteins in each group (n = 3). d RNA fluorescence probe detection of the effect of ALYREF-Cas9 on Nrf2 mRNA (n = 3, scale bar = 10 µm). e RNA fluorescence probe detection of the effect of ALYREF-Cas9 and NSUN2 overexpression on Nrf2 mRNA (n = 3, scale bar = 10 µm). The data is represented by the average ± SD of three trials, and compared with the control group, *P < 0.05, **P < 0.01, ***P < 0.001