Fig. 2

The effect of circHIF1A on CRC cell function (Cetuximab 5 µg/mL). A-D. The expression levels of Ki67 (A) and EdU (B) in LIM1215-R decreased after circHIF1A downregulation, while those in LIM1215 increased after circHIF1A overexpression (C-D). E-F. The effect of circHIF1A downregulation (E) and overexpression (F) on apoptosis levels in LIM1215-R and LIM1215, respectively, as detected by flow cytometry. G-H. The effect of circHIF1A levels on the cell cycle. I-J. Changes in aerobic metabolism (I) and glycolytic metabolism (J) in LIM1215-R after circHIF1A downregulation. K-L. Changes in aerobic metabolism (K) and glycolytic metabolism (L) in LIM1215 after circHIF1A overexpression. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001