Fig. 1

Morphology and immunophenotyping of hLC-MSCs. A (a) Representative images of primary cells arising from NSCLC tissues. Magnification, ×40. (b–d) The resultant distinct, purified colonies of epithelial tumors cells (b) or hLC-MSCs (c–d). Magnification ×200. B Representative images of hLC-MSC clones that were subjected to immunocytochemical staining. The hLC-MSCs were negative for E-cadherin and positive for N-cadherin, vimentin, α-SMA, and CK18 expression. Cytoskeletal proteins were stained using phalloidin, and cell nuclei were stained with DAPI. hLC-MSCs human lung cancer-derived mesenchymal stem cells; NSCLC non-small cell lung cancer; α-SMA alpha smooth muscle actin; CK18 cytokeratin 18; DAPI 4’,6-diamidino-2-phenylindole