Fig. 2

SFMBT2 suppressed proliferation and growth of clear cell RCC cells. A MTT assay was applied for measure the viability of clear cell RCC cells with SFMBT2 overexpression. B Plate colony formation assay was performed to assess the colony formation capacity of 786-O and 769-P cells. C The cell cycle was detected by flow cytometry. D Cell cycle-related protein, cyclin E, cyclin D and p21 were determined by western blot. E The 786-O cells with increased expression of SFMBT2 were inoculated into nude mice, and the subcutaneous tumors were shown (the scale bar presented as 1 cm). F The tumor growth curve. G The tumor weight. H Immunohistochemistry staining was performed to determine the expression of SFMBT2 and Ki-67 in the tumors (the scale bar presented as 50 μm). *p < 0.05;**p < 0.01; ***p < 0.001 versus LV-NC