Fig. 4

The enhanced expression of SFMBT2 inhibited migration, invasion and metastasis of clear cell RCC cells. A, B Wound healing assay was carried out to examine the migratory ability of clear cell RCC cells with SFMBT2 overexpression (the scale bar presented 200 μm). C, D Transwell assay supplemented with matrigel was used to detect the invasion of clear cell RCC cells (the scale bar presented 100 μm). E The GFP-stably-expressed 786-O cells were intravenously injected into nude mice, and the in vivo bioluminescence imaging was done. The lung was isolated from mice, and the nodule numbers in lung were counted. F HE staining was performed to measure the pathomorphological changes of lung (the scale bar presented 50 μm). *p < 0.05; **p < 0.01; ***p < 0.001 versus LV-NC