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Fig. 8 | Biology Direct

Fig. 8

From: Acetylation- and ubiquitination-regulated SFMBT2 acts as a tumor suppressor in clear cell renal cell carcinoma

Fig. 8

HDAC3 enhanced the ubiquitination of SFMBT2 mediated by SIAH1. A The structure of SFMBT2 protein and three potential acetylation/ubiquitination sites (K504, K505 and K687) from AlphaFold database (https://alphafold.ebi.ac.uk/) were shown. B Three SFMBT2 mutant vectors (K504A, K505A and K687A) were constructed. C These three mutant vectors were transfected into 293T cells, and the expression was verified by western blot. D The acetylated SFMBT2 levels were measured in 293T cells after RGFP966 treatment. E The binding between SIAH1 an SFMBT2 was confirmed by IP. F The SIAH1 expression plasmid was transfected into clear cell RCC cells, and the protein levels of SIAH1 and SFMBT1 were determined. G The ubiquitination levels of SFMBT2 after SIAH1 overexpression were detected. H The ubiquitination levels of SFMBT2 wild type or K687A mutant type were examined after SIAH1 overexpression. I The ubiquitination levels of SFMBT2 after overexpression of SIAH1 and HDAC3. J The ubiquitination levels of SFMBT2 in 293T cells with SIAH1 overexpression and RGFP966 application

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