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Fig. 6 | Biology Direct

Fig. 6

From: YY1 mediated DCUN1D5 transcriptional activation promotes triple-negative breast cancer progression by targeting FN1/PI3K/AKT pathway

Fig. 6

DCUN1D5 promotes TNBC progression by activating FN1/PI3K/AKT pathway. A The mRNA expression of FN1 was assessed by qRT-PCR with DCUN1D5 depletion or overexpression. B The protein expression of FN1, p-PI3K, PI3K, p-AKT and AKT with DCUN1D5 knockdown was evaluated by western blot. C IHC staining of FN1, p-PI3K and p-AKT in xenograft tumors (×400). Scale bar: 25 μm. D The protein expression of p-PI3K, PI3K, p-AKT and AKT was assessed by western blot with DCUN1D5 knockdown and FN1 overexpression or IGF-1 treatment. E, F Cell proliferation, migration and invasion ability was assessed by CCK-8 and transwell assay with DCUN1D5 knockdown and FN1 overexpression (IGF-1 treatment). ** p < 0.01, *** p < 0.001, #p < 0.05 (shDCUN1D5 vs. shDCUN1D5 + FN1), ###p < 0.001 (shDCUN1D5 vs. shDCUN1D5 + IGF-1)

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