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Fig. 6 | Biology Direct

Fig. 6

From: METTL3 promotes the progression of osteosarcoma through the N6-methyladenosine modification of MCAM via IGF2BP1

Fig. 6

METTL3 deficiency inhibited OS cell progression by altering MCAM expression. MG63 and U2OS cells were transfected with sh-NC, sh-METTL3, sh-METTL3 + pcDNA or sh-METTL3 + MCAM. (A) MCAM protein level in MG63 and U2OS cells was measured via western blot. (B) MG63 and U2OS cell viability was assessed by MTT assay. (C) The colony formation of MG63 and U2OS cells was estimated by colony formation assay. (D and E) The apoptosis of MG63 and U2OS cells was analyzed by flow cytometry analysis. (F and G) The invasion and migration of MG63 and U2OS cells were evaluated by transwell assay and wound-healing assay, respectively. (H and I) The protein levels of CyclinD1 and MMP9 in MG63 and U2OS cells were detected by western blot. *P < 0.05, **P < 0.01, ***P < 0.001

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Biology Direct

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