Skip to main content
Fig. 8 | Biology Direct

Fig. 8

From: METTL3 promotes the progression of osteosarcoma through the N6-methyladenosine modification of MCAM via IGF2BP1

Fig. 8

YY1 promoted the transcriptional regulation of METTL3 in OS cells. (A) Jaspar database predicted binding sites between transcription factor YY1 and METTL3 promoter. (B) ChIP assay was performed the combination between YY1 and METTL3. (C) The protein expression of YY1 in MG63 and U2OS cells transfected with si-NC, si-YY1, pcDNA or YY1 was determined by western blot. (D and E) The luciferase activity in MG63 with pcDNA/YY1 and WT-METTL3/MUT-METTL3 co-transfection and U2OS cells with si-NC/si-YY1 and WT-METTL3/MUT-METTL3 co-transfection was examined through dual-luciferase reporter assay. (F) METTL3 protein level in MG63 and U2OS cells transfected with si-NC, si-YY1, pcDNA or YY1 was measured via western blot. **P < 0.01, ***P < 0.001

Back to article page

Biology Direct

ISSN: 1745-6150

Contact us