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Fig. 2 | Biology Direct

Fig. 2

From: Hypoxia-induced activation of HIF-1alpha/IL-1beta axis in microglia promotes glioma progression via NF-κB-mediated upregulation of heparanase expression

Fig. 2

Hypoxia treatment stimulates IL-1β secretion by microglia via activating HIF-1α. (A) The TNF-α, MCAF, IL-6, IL-1α, INF-γ, IL-1β, IL-8, and GM-CSF levels in HMC3 culture medium were measured by an ELISA kit. (B and C) The abundance of HIF-1α protein in HMC3 cells was detected by western blot. (D) The abundance of IL-1β in HMC3 cells was detected by western blot. (E) The IL-1β level in HMC3 culture medium was detected by an ELISA kit. n = 3. *P < 0.05, ***P < 0.001

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