Fig. 6

SNHG20 regulates the ubiquitination and stability of β-catenin. (A) U2932 and OCI-LY3 cells with SNHG20 knockdown were treated with MG132 (10 µM). The protein level of β-catenin was measured using western blotting. (B) U2932 and OCI-LY3 cells with or without SNHG20 knockdown were treated with CHX (40 µg/mL). The protein level of β-catenin was measured using western blotting at different time points. (C) U2932 and OCI-LY3 cells were transduced with shNT or shSNHG20. Immunoprecipitation was performed using an anti-β-catenin antibody, and the protein levels of Ub and β-catenin were measured via western blotting. (D) U2932 cells were transduced with shNT or shSNHG20. Immunoprecipitation was performed using an anti-β-catenin antibody, and the protein levels of USP14 and β-catenin were measured via western blotting. (E) HEK293T cells were transfected with the corresponding vectors. Immunoprecipitation was performed using an anti-HA antibody, and the protein levels of Flag-USP14 and HA-β-catenin were measured via western blotting. *P < 0.05