Fig. 6
From: The deubiquitinating protein OTUD6B promotes lung adenocarcinoma progression by stabilizing RIPK1
OTUD6B can bind to RIPK1, reduce its ubiquitination level and increase its protein stability. A The binding localization of the OTUD6B protein to RIPK1 was detected by fluorescence in situ hybridization (FISH) combined with an in situ proximity ligation assay (PLA). B, C Cycloheximide (CHX) was used to detect the effect of OTUD6B interference or mutation on the degradation rate of RIPK1 via Western blotting and qPCR. D–F The effect of OTUD6B interference on the expression level of the RIPK1 protein was verified by Western blot experiments