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Fig. 4 | Biology Direct

Fig. 4

From: Silencing LINC00987 ameliorates adriamycin resistance of acute myeloid leukemia via miR-4458/HMGA2 axis

Fig. 4

LINC00987 overexpression enhanced the cell viability, clone formation and ADR-resistance of AML cells. (A) LINC00987 expression in MOLM13 and HL-60 cells were measured by RT-qPCR after transfection with pcDNA-LINC00987 and pcDNA-Empty at 24 h. (B) The proliferation of MOLM13 and HL-60 cells was detected by CCK8 after transfection with pcDNA-LINC00987 and pcDNA-Empty at 24 h under 0.2 µM ADR treatment. Then, the cell viability was calculated according to proliferation. (C) Clone formation was detected by soft agar assay colony formation. The number of cell clone formations was counted by imaging at 100x magnification. (D) The IC50 of ADR for the pcDNA-LINC00987-transfected MOLM13 and HL-60 cells were shown after different concentrations of ADR treatment at 24 h. (E) Under 0.2 µM ADR treatment, P-GP and BCRP expression in MOLM13 and HL-60 cells were measured by Western blot. (F) Apoptosis was detected by TUNEL staining and imaging at 400x magnification. ***P < 0.001

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