Fig. 6

HMGA2 promotes cell viability and ADR-resistance to assist in LINC00987 function in ADR-resistant AML cells. (A) HMGA2 protein expression in ADR/MOLM13 and ADR/HL-60 cells were measured by Western blot after si1-LINC00987 and pcDNA-HMGA2 co-transfection at 24 h. (B) The proliferation of ADR/MOLM13 and ADR/HL-60 cells was detected using CCK8 after co-transfection at 24 h under 0.2 µM ADR treatment. Then, the cell viability was calculated according to proliferation. (C) Clone formation of ADR/MOLM13 and ADR/HL-60 cells was detected by soft agar colony formation assay after co-transfection at 24 h under 0.2 µM ADR treatment. The number of cell clone formations was counted by imaging at 100× magnification. (D) The IC50 of ADR for co-transfected ADR/MOLM13 and ADR/HL-60 cells were shown after different concentrations of ADR treatment at 24 h. (E) Under 0.2 µM ADR treatment, P-GP and BCRP expression in ADR/MOLM13 and ADR/HL-60 cells were measured by western blotting after si1-LINC00987 and pcDNA-HMGA2 co-transfection. ***P < 0.001