Skip to main content
Fig. 2 | Biology Direct

Fig. 2

From: PNSC928, a plant-derived compound, specifically disrupts CtBP2-p300 interaction and reduces inflammation in mice with acute respiratory distress syndrome

Fig. 2

Proinflammatory cytokine genes were downregulated in knockdown cell lines of CtBP2-p300-NF-κB complex members. (A) Experimental design: RNA-Seq analysis to DGEs in LPS-challenged ControlKD, CtBP2KD, p300KD, and p65KD cells. (B) Venn diagram illustrating the altered gene profiles in LPS-challenged ControlKD, CtBP2KD, p300KD, and p65KD cells. (C) Heatmap displaying the top 7 upregulated genes and top 11 downregulated genes in LPS-challenged ControlKD, CtBP2KD, p300KD, and p65KD cells. (D-F) Verification of 12 DGEs by RT-qPCR analysis (n = 3). (D) IL-1B, IL-6, IL-15, and IL-18; (E) TNFA, IFNG, S100A8, and S100A9; (F) LCP1, NME2, CDH1, and SOCS1. *P < 0.05; **P < 0.01; ***P < 0.001

Back to article page

Biology Direct

ISSN: 1745-6150

Contact us