Fig. 7
From: Single-cell transcriptomic profiling unveils insights into ovarian fibrosis in obese mice
Verification of key genes expression pattern. a Immunohistochemical staining of SPP1 and TNF-α in ovarian tissue in the control group and the obese group. b Immunofluorescence staining of CD44 and macrophage marker F4/80 in ovarian tissue in the control group and the obese group. Arrows indicate cells expressing both F4/80 and CD44. Quantitative PCR analysis of the mRNA expression of CD44, TNF-α, IL 6 and IL-1β after treatment with indicated concentration of SPP1 recombinant protein for 24 h in RAW264.7 cell (c) or THP-1 cell (d). Western blot analysis of the expression of CD44 protein after treatment with indicated concentration of SPP1 recombinant protein for 24 h in RAW264.7 cell (e) or THP-1 cell (f). g Detection of SPP1 level in follicular fluid of obese and NW women. h Determine of SPP1 expression in granulosa cell culture supernatant of obese and NW women. (n.s, not significant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001)