Fig. 2

RBM15 promoted DDP resistance in LC cells through inhibition of ferroptosis. After treatment of drug-resistant cells with 10 µg/mL DDP, (A): Iron content in cells was measured by colorimetric assay; (B): Intracellular ROS levels were measured using the DCFH-DA probe; (C): MDA levels in cells were assessed using a lipid peroxidation assay kit; (D): GSH content in cells was evaluated using a GSH assay kit; (E-F): The expression of ACSL4 and GPX4 in cells was detected by Western blot. Drug-resistant cells were treated with the ferroptosis inhibitor Fer-1, with DMSO treatment as a negative control, and then (G): Cell inhibition rate of LC cancer cell lines treated with DDP was measured by CCK-8 assay; (H): The number of cell clones was measured by colony formation assay. The experiments were independently repeated three times, and the data are expressed as mean ± standard deviation. Data comparisons among multiple groups were analyzed using two-way ANOVA, followed by Tukey’s multiple comparisons test. * p < 0.05, ** p < 0.01