Fig. 1

PDE5 Inhibitor Tadalafil Suppresses Proliferation and FMT in Prostate Stromal Cells. (A) WPMY-1 cells were subjected to various concentrations of tadalafil treatment for 1 week, and cellular proliferation vitality was assessed on 0, 1, 3, 5, and 7 days. (B) WPMY-1 cells were exposed to various concentrations of tadalafil for 48 h, and alterations in cell proliferation were evaluated by EdU incorporation assay. (C-D) After a 72-hour treatment of PrFc or WPMY-1 cells with 1 ng/mL TGFβ1, a continued 48-hour exposure to 25 µM tadalafil was administered, and the mRNA expression levels of αSMA and IGFBP3 were quantified using qRT-PCR. (E-F) Following a 72-hour activation of PrFc and WPMY-1 cells with 1 ng/mL TGFβ1, subsequent treatment with 25 µM tadalafil for 48 h was carried out, and the expression levels of proteins related to the TGFβ pathway were examined. (G-H) Immunofluorescence staining was employed to analyze levels of Vimentin and αSMA expression in PrFc and WPMY-1 cells following a 48-hour treatment with 25µM tadalafil or TGFβ1. (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001)