Fig. 4

CNBP regulates the stemness transition of ovarian cancer cells (A) Sphere formation of OCSC overexpressing CNBP, and percentage of CD133⁺/CD117⁺ cells overexpressing CNBP to total cells detected by flow cytometry. (B) Effects of CNBP on cell proliferation detected by using CCK-8 assay: a. Overexpression of CNBP in OCSCs; b. Inhibition of CNBP expression in COC1 and SKOV3 cells. C. Differences in the inhibition rate of each group of cells treated with different concentrations of cisplatin after modulating the expression of CNBP: The cells of each group were added with different concentrations of cisplatin (0, 10, 30, 50 µM) and then continued to be cultured for 48 h. The effect of different concentrations of cisplatin on the inhibition rate of each group of cells after regulating the expression of CNBP was detected by using CCK-8 assay: a. OCSC overexpressing CNBP; b and c. Suppression of CNBP expression in COC1 and SKOV3 cells. Detection of cell biological behaviors after modulating CNBP expression in each group of cells: overexpression of CNBP in OCSC and inhibition of CNBP expression in COC1 and SKOV3 cells. D. Cell migration and invasion were measured by transwell assays. E. Colony formation assays for each group of cells; F. Cell cycle percentage assays for each group of cells; G. Apoptotic percentage assays for each group of cells; H. Detection of relevant protein expression in each group of cells by western blotting. I. The effect of CNBP on the activity of the Wnt pathway in each group of cells was detected. The relative fluorescence activity of each group of cells was assayed after transfection with TOPFlash/FOPFlash vectors: a. OCSC overexpressing CNBP; b. COC1 and SKOV3 cells with suppressed CNBP expression, *P < 0.05