Fig. 5

LINC00665 promotes stemness transition of ovarian cancer cells by mediating CNBP expression A. The effects of CNBP and LINC00665 expression regulation on cell proliferation were detected using CCK-8 assays: a. LINC00665 and CNBP expression was modulated in OCSC; b. LINC00665 and CNBP expression was modulated in COC1 and SKOV3 cells. B. Differences in the inhibition rate of cells in each group with a modulated expression of LINC00665 and CNBP by different concentrations of cisplatin were detected: cells in each group were added with different concentrations of cisplatin (0, 10, 30, and 50 µM) and then continued to be cultured for 48 h. The inhibition rate of (a) OCSCs, (b) COC1 cells, and (c) SKOV3 cells with modulated expression of LINC00665 and CNBP by different concentrations of cisplatin was detected by using CCK-8 assays. The cell biological behaviors of each group of cells after modulating the expression of CNBP and LINC00665 were detected: the expression of LINC00665 and CNBP was co-repressed in OCSC and co-overexpressed in COC1 and SKOV3 cells. C. Cell migration and invasion were measured by transwell assays. D. Colony formation assays. E. Cell cycle percentage assays. F. Detection of apoptotic percentages. G. Detection of relevant protein expression by western blotting. H. Detection of the effect of LINC00665 and CNBP on the activity of the Wnt pathway. The relative fluorescence activity of each group of cells was assayed after transfection with TOPFlash/FOPFlash vectors: a. OCSCs with suppressed expression of LINC00665 and CNBP; b. COC1 and SKOV3 cells overexpressing LINC00665 and CNBP, *P < 0.05