Table 3 Antibodies used in immunofluorescence staining
From: STAU1-mediated CNBP mRNA degradation by LINC00665 alters stem cell characteristics in ovarian cancer
Rabbit monoclonal antibody Lgr5 | 1:100 | Novus Biologicals, MAB8078-SP, Centennial, CO, USA | 4 °C overnight |
|---|---|---|---|
Rabbit polyclonal antibody ALDH1 | 1:300 | Wanleibio, WL02762 | 4 °C overnight |
Rabbit monoclonal antibody SOX2 | 1:100 | Abclonal, A0561 | 4 °C overnight |
Rabbit monoclonal antibody OCT4 | 1:100 | Affinity Biologicals, AF0226, Shanghai, China | 4 °C overnight |
Rabbit monoclonal antibody Nanog | 1:100 | Affinity, AF5388 | 4 °C overnight |
FITC-conjugated goat anti-rabbit IgG | 1:100 | Abcam, ab6717 | Room temperature, 1Â h |
Cy3-conjugated goat anti-mouse IgG | 1:200 | Invitrogen, A-21,424 | Room temperature, 1Â h |