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Fig. 3 | Biology Direct

Fig. 3

From: METTL3 in cancer-associated fibroblasts-derived exosomes promotes the proliferation and metastasis and suppresses ferroptosis in colorectal cancer by eliciting ACSL3 m6A modification

Fig. 3

m6A profiles and METTL3 expression were increased in CAFs and CRC cells. (A) The m6A contents were in LoVo and HCT-116 cells after CAF-CM incubation using the RNA m6A quantification kit. (B, C) Western blotting analysis for METTL3 and METTL14 levels in LoVo and HCT-116 cells after NF-CM or CAF-CM incubation. (D) Western blotting analysis for METTL3 expression in NF-CM or CAF-CM. (E, F) TCGA and ENCORI databases showed that METTL3 levels were higher in colon cancer tissues compared with the normal tissues (G, H) qRT-PCR and western blotting analyses for METTL3 levels in CRC tissues and matched normal tissues. I Western blotting for METTL3 protein levels in CRC cell lines and NCM460 normal cells. Technical replicates (n = 3), biological replicates (n = 3), *P < 0.05

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