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Fig. 5 | Biology Direct

Fig. 5

From: METTL3 in cancer-associated fibroblasts-derived exosomes promotes the proliferation and metastasis and suppresses ferroptosis in colorectal cancer by eliciting ACSL3 m6A modification

Fig. 5

Knockdown of METTL3 in CAFs-exo suppresses CRC cell proliferation invasion and migration and induces cell ferroptosis. (A) CAFs were transfected with sh-METTL3 or sh-NC, and levels of METTL3 in CAFs were examined by western blotting. (B) Exosomal METTL3 levels in CAFs transfected with sh-METTL3 or sh-NC were measured using western blotting. (C-N) LoVo and HCT-116 cells were incubated with sh-METTL3-CAFs-exo or sh-NC-CAFs-exo for 24 h. (C) Western blotting analysis for METTL3 expression in CRC cells. (D-F) MTT and colony formation assays for cell proliferation analysis. (G-I) Detection analysis of cell migration and invasion using transwell and wound healing assays. (J, K) Western blotting analysis for PCNA and MMP9 protein levels. (L-N) The accumulation of ROS and Fe2+, and the exhaustion of GSH were detected using commercial kits. Technical replicates (n = 3), biological replicates (n = 3), *P < 0.05

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