Fig. 4

ADAMTS4 inhibited ubiquitination of c-Myc through WWP2. A Venn diagram indicated the potential E3 ubiquitin ligase of c-Myc that may interact with ADAMTS4. B Co-IP assays showed endogenous interaction between ADAMTS4, WWP2 and c-Myc in A549 and NCI-H1299 cells. (n = 3). C WB analysis showed c-Myc expression in control or WWP2-overexpressed A549 and NCI-H1299 cells treated with CHX. (n = 3). D Western blot analysis was performed to assess the protein levels of c-Myc in control and WWP2-overexpressed A549 and NCI-H1299 cells, with or without MG132 treatment. (n = 3). E Western blot analysis was conducted to evaluate the ubiquitination levels of c-Myc in A549 and NCI-H1299 cells overexpressing ADAMTS4 alone or co-overexpressing ADAMTS4 and WWP2 simultaneously. (n = 3)