Fig. 2

Analysis of Glucose Metabolism in HSCs under Lung Cancer Conditions. Note A Comparison of glucose uptake between normal HSCs (N-HSCs) and lung cancer HSCs (T-HSCs) using 2-NBDG as a fluorescent probe (n = 3). B Measurement of lactate concentration showing a significant increase in lactic acid production per thousand cells in T-HSCs (n = 3; **p < 0.01). C Determination of Acetyl-CoA concentration indicating potential enhancement of oxidative phosphorylation pathway in T-HSCs (n = 3; *p < 0.05). D Assessment of ATP concentration revealing increased metabolic activity in T-HSCs (n = 3; *p < 0.05). E–F The measurement of the oxygen consumption rate (OCR) and the extracellular acidification rate (ECAR) of LT-HSCs using the Seahorse equipment; G Cellular NADH representative spectra: Flow cytometry profiles before and after treatment with antimycin A (AMA) (n = 3); H Quantitative comparison of NADH accumulation induced by antimycin A; (I) Detection of the survival rate of T-HSCs using AZD3965 via CCK8 assay (n = 3; *p < 0.05)