Fig. 1
Serum metabolomics reveals different metabolic profiles between normal subjects and patients with GTN. A The OPLS-DA score plot for GTN and normal. B Volcano plot of DMs. C Heatmap of DMs in normal and GTN groups. Log2FC were calculated to show the differences in levels of metabolites. Those with P < 0.05, VIP ≥ 1, and Log2FC > 1.5 were considered as the significant DMs between two groups. The threshold of VIP was set to 1. D KEGG enrichment analysis of the biological pathways of DMs. E The 24 identified DMs ranked by their contributions and shown as VIP scores. F A receiver operating characteristics (ROC) curve for Equol level prediction. DMs differential metabolites, GTN gestational trophoblastic neoplasia, VIP variable importance in the projection, OPLS-DA orthogonal projection to latent structures-discriminant analysis