Fig. 3

Effects of Equol on choriocarcinoma cell apoptosis. JEG-3 and Bewo cells were treated with Equol (20 or 40 μM) or vehicle for 24 h, and then subjected to A Annexin V-FITC/PI apoptosis assays. B The expression of apoptosis-related proteins, including cleaved caspase-3, cleaved caspase-7, cleaved caspase-9, and cleaved PARP, was determined by western blot. C and D The activity of caspase-3 was measured. E JC-1 staining was performed to evaluate the mitochondria membrane potential of cells. F Cytoplasm and mitochondria fraction were subjected to western blot to detect Cyt C. Veh vehicle, Cyt C cytochrome C