Fig. 4

Screening of the proteins regulated by Equol in choriocarcinoma cells. A The PCA plot for the similarity assessment of samples from the Veh. group and the Equol group. B The volcano plot showing the DEPs in the Equol group when compared with the Veh. group. The proteins with P < 0.05 and Log₂FC > 1 were considered as the significant DEPs between two groups. C The sankey diagram combined with a bubble diagram visualized the significantly enriched GO terms associated with protein degradation and the proteins annotated within. D The heatmap visualized the expression patterns of DEPs, and the proteins enriched in the GO terms associated with protein degradation were highlighted. E The binding mode of Equol to TRIM21 by molecule docking. F JEG-3 and Bewo cells were treated with Equol (20 or 40 μM) or vehicle for 24 h. The expression of TRIM21 was measured by western blot. PCA principal component analysis, Veh. vehicle