Fig. 5

Roles of TRIM21 in the effect of Equol on choriocarcinoma cell proliferation and apoptosis. A JEG-3 and Bewo cells were transfected with siTRIM21 or siNC for 48 h. The expression levels of TRIM21 mRNA and protein were measured by qRT-PCR and western blot. At 48 h after cell transfection, the cells were treated with 40 μM Equol for 24 h. B Cell viability was detected by CCK-8 assay. (C) Cell apoptosis was determined by flow cytometry. Veh. vehicle