Fig. 7

Roles of ANXA2 in the effect of TRIM21 on the proliferation and apoptosis of Equol-treated choriocarcinoma cells. A JEG-3 and Bewo cells were transfected with siANXA2 or siNC for 48 h. The expression levels of ANXA2 mRNA and protein were measured by qRT-PCR and western blot. JEG-3 and Bewo cells were co-transfected with siTRIM21 and siANXA2 for 48 h, and then treated with 40 μM Equol for 24 h. B Cell viability was detected by CCK-8 assay. C Cell apoptosis was determined by flow cytometry. Veh. vehicle