Fig. 8

Roles of TRIM21 and AXNA2 in Equol-induced effects on β-catenin activation. A JEG-3 and Bewo cells were co-transfected with TOPflash-luc and pRL-TK and treated with 20 or 40 μM Equol for 48 h. B The siTRIM21 or siNC was co-transfected with TOPflash-luc and pRL-TK into JEG-3 and Bewo cells for 48 h, and then the cells treated with 40 μM Equol for 24 h. C JEG-3 and Bewo cells were co-transfected with siTRIM21, siANXA2, TOPflash-luc, and pRL-TK for 48 h, and then treated with 40 μM Equol for 24 h. A–C The relative activity of luciferase was measured. Veh. vehicle. D Schematic illustration of the mechanism of β-catenin activation regulated by Equol