Fig. 2

Overexpression of hsa_circ_0020093 represses OC progression in vitro and in vivo. A The effect of hsa_circ_0020093 overexpression on SKOV3 and OVCAR3 cell viability was determined by CCK8 assay. P value was calculated by two-way ANOVA. B–C The effects of hsa_circ_0020093 overexpression on cell migration and invasion ability of SKOV3 and OVCAR3 cells were assessed by transwell assays with or without matrigel. Scale bar: 200 μm. P value was calculated by two-tailed Student’s t-test. D–E Flow cytometry was used to detect cell apoptosis, APC( +)/PI( −) indicates early apoptosis while APC( +)/PI( +) indicates late apoptosis. F Western blot analysis of caspase-3 and cleaved caspase-3 expression after overexpression of hsa_circ_0020093 in SKOV3 and OVCAR3 cells. The activity of caspase-3 was calculated by cleaved caspase-3/total caspase-3. G RT-qPCR analysis of the RNA level of hsa_circ_0020093 and ATRNL1 mRNA in SKOV3 cells stably overexpressing hsa_circ_0020093 and control. P value was calculated by two-tailed Student’s t-test. H Xenograft tumors of hsa_circ_0020093-overexpressing and control SKOV3 cells (n = 6). I The xenograft tumor growth of hsa_circ_0020093-overexpressing and control SKOV3 cells. P value was calculated by two-way ANOVA. J The xenograft tumor weight of hsa_circ_0020093-overexpressing and control SKOV3 cells. P value was calculated by two-tailed Student’s t-test. K The representative images of tumor tissues stained with Ki67 (Left). L Ki67-positive degree in tumor tissues was calculated by immunohistochemistry (IHC) scores. Scale bar: 100 μm. P value was calculated by two-tailed Student’s t test. ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001