Fig. 4

hsa_circ_0020093 regulates mitochondrial mRNA expression and mitochondria abundance at least partially by inhibiting LRPPRC activity. A RT-qPCR analysis of the abundance of 13 mitochondrial mRNAs following co-transfection of hsa_circ_0020093-overexpressing or control and LRPPRC-overexpressing or pcDNA (control) plasmids. P value was calculated by one-way ANOVA. B The morphology and abundance of mitochondria were observed under transmission electron microscopy. Scale bar: 5 μm, 2 μm and 500 nm respectively. C The mitochondria number per cell in SKOV3 cells with stable transfection of control and hsa_circ_0020093. P value was calculated by two-tailed Student’s t-test. *P < 0.05; **P < 0.01