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Fig. 7 | Biology Direct

Fig. 7

From: GALNT9 enrichment attenuates MPP+-induced cytotoxicity by ameliorating protein aggregations containing α-synuclein and mitochondrial dysfunction

Fig. 7

Evaluation of mitophagy and PCD involved in CytC-associated apoptotic pathway. (A) Evaluation of molecules in CytC-associated apoptotic pathway by western blot assay. M, PageRuler prestained protein ladder (Yamei WJ103). Protein bands were normalized to GAPDH levels. Examinations was performed in triplicate at least. (B) Statistical analysis of expression of molecules including CytC, Apaf1, CASP9, and CASP3, respectively. Red asterisks represent comparison between GALNT9-regulated groups and controls, and black asterisks represent comparison between MPP+-treated and -untreated groups. *, P < 0.05; **, P < 0.01; ***, P < 0.001. (C) Dual immunofluorescence staining of mitophagy. The dimension of micrographs was 1,731.43 mm × 1,151.47 mm. Green signals correspond to lysosomes. Red signals correspond to mitochondria. Mitochondrial fission is depicted as the juxtaposed images of green and red: dual immunolabelling of mitochondria/lysosomes is visible as orange or yellow indicated by white tips

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